This class represents the CIGAR without any methods to set the cigar (see CigarRoller for that). More...
#include <Cigar.h>
Classes | |
struct | CigarOperator |
Public Types | |
enum | Operation { none = 0, match, mismatch, insert, del, skip, softClip, hardClip, pad } |
Enum for the cigar operations. More... | |
Public Member Functions | |
Cigar () | |
Default constructor initializes as a CIGAR with no operations. | |
void | getCigarString (String &cigarString) const |
Set the passed in String to the string reprentation of the Cigar operations in this object. | |
void | getCigarString (std::string &cigarString) const |
Set the passed in std::string to the string reprentation of the Cigar operations in this object. | |
void | getExpandedString (std::string &s) const |
Sets the specified string to a valid CIGAR string of characters that represent the cigar with no digits (a CIGAR of "3M" would return "MMM"). | |
const CigarOperator & | operator[] (int i) const |
Return the Cigar Operation at the specified index (starting at 0). | |
const CigarOperator & | getOperator (int i) const |
Return the Cigar Operation at the specified index (starting at 0). | |
bool | operator== (Cigar &rhs) const |
Return true if the 2 Cigars are the same (the same operations of the same sizes). | |
int | size () const |
Return the number of cigar operations. | |
void | Dump () const |
Write this object as a string to cout. | |
int | getExpectedQueryBaseCount () const |
Return the length of the read that corresponds to the current CIGAR string. | |
int | getExpectedReferenceBaseCount () const |
Return the number of bases in the reference that this CIGAR "spans". | |
int | getNumBeginClips () const |
Return the number of clips that are at the beginning of the cigar. | |
int | getNumEndClips () const |
Return the number of clips that are at the end of the cigar. | |
int32_t | getRefOffset (int32_t queryIndex) |
Return the reference offset associated with the specified query index or INDEX_NA based on this cigar. | |
int32_t | getQueryIndex (int32_t refOffset) |
Return the query index associated with the specified reference offset or INDEX_NA based on this cigar. | |
int32_t | getRefPosition (int32_t queryIndex, int32_t queryStartPos) |
Return the reference position associated with the specified query index or INDEX_NA based on this cigar and the specified queryStartPos which is the leftmost mapping position of the first matching base in the query. | |
int32_t | getQueryIndex (int32_t refPosition, int32_t queryStartPos) |
Return the query index or INDEX_NA associated with the specified reference offset when the query starts at the specified reference position. | |
int32_t | getExpandedCigarIndexFromQueryIndex (int32_t queryIndex) |
Returns the index into the expanded cigar for the cigar associated with the specified queryIndex. | |
int32_t | getExpandedCigarIndexFromRefOffset (int32_t refOffset) |
Returns the index into the expanded cigar for the cigar associated with the specified reference offset. | |
int32_t | getExpandedCigarIndexFromRefPos (int32_t refPosition, int32_t queryStartPos) |
Returns the index into the expanded cigar for the cigar associated with the specified reference position and queryStartPos. | |
char | getCigarCharOp (int32_t expandedCigarIndex) |
Return the character code of the cigar operator associated with the specified expanded CIGAR index. | |
char | getCigarCharOpFromQueryIndex (int32_t queryIndex) |
Return the character code of the cigar operator associated with the specified queryIndex. | |
char | getCigarCharOpFromRefOffset (int32_t refOffset) |
Return the character code of the cigar operator associated with the specified reference offset. | |
char | getCigarCharOpFromRefPos (int32_t refPosition, int32_t queryStartPos) |
Return the character code of the cigar operator associated with the specified reference position. | |
uint32_t | getNumOverlaps (int32_t start, int32_t end, int32_t queryStartPos) |
Return the number of bases that overlap the reference and the read associated with this cigar that falls within the specified region. | |
bool | hasIndel () |
Return whether or not the cigar has indels (insertions or delections). | |
Static Public Member Functions | |
static bool | foundInReference (Operation op) |
Return true if the specified operation is found in the reference sequence, false if not. | |
static bool | foundInReference (char op) |
Return true if the specified operation is found in the reference sequence, false if not. | |
static bool | foundInReference (const CigarOperator &op) |
Return true if the specified operation is found in the reference sequence, false if not. | |
static bool | foundInQuery (Operation op) |
Return true if the specified operation is found in the query sequence, false if not. | |
static bool | foundInQuery (char op) |
Return true if the specified operation is found in the query sequence, false if not. | |
static bool | foundInQuery (const CigarOperator &op) |
Return true if the specified operation is found in the query sequence, false if not. | |
static bool | isClip (Operation op) |
Return true if the specified operation is a clipping operation, false if not. | |
static bool | isClip (char op) |
Return true if the specified operation is a clipping operation, false if not. | |
static bool | isClip (const CigarOperator &op) |
Return true if the specified operation is a clipping operation, false if not. | |
static bool | isMatchOrMismatch (Operation op) |
Return true if the specified operation is a match/mismatch operation, false if not. | |
static bool | isMatchOrMismatch (const CigarOperator &op) |
Return true if the specified operation is a match/mismatch operation, false if not. | |
Static Public Attributes | |
static const int | MAX_OP_VALUE = pad |
static const int32_t | INDEX_NA = -1 |
Value associated with an index that is not applicable/does not exist, used for converting between query and reference indexes/offsets when an associated index/offset does not exist. | |
Protected Member Functions | |
void | clearQueryAndReferenceIndexes () |
void | setQueryAndReferenceIndexes () |
Protected Attributes | |
std::vector< CigarOperator > | cigarOperations |
Friends | |
std::ostream & | operator<< (std::ostream &stream, const Cigar &cigar) |
Writes all of the cigar operations contained in the cigar to the passed in stream. |
This class represents the CIGAR without any methods to set the cigar (see CigarRoller for that).
This class represents the CIGAR. It contains methods for converting to strings and extracting information from the cigar on how a read maps to the reference.
It only contains read only methods. There are no ways to set values. To set a value, a child class must be used.
Definition at line 83 of file Cigar.h.
enum Cigar::Operation |
Enum for the cigar operations.
Definition at line 87 of file Cigar.h.
00087 { 00088 none=0, ///< no operation has been set. 00089 match, ///< match/mismatch operation. Associated with CIGAR Operation "M" 00090 mismatch, ///< mismatch operation. Associated with CIGAR Operation "M" 00091 insert, ///< insertion to the reference (the query sequence contains bases that have no corresponding base in the reference). Associated with CIGAR Operation "I" 00092 del, ///< deletion from the reference (the reference contains bases that have no corresponding base in the query sequence). Associated with CIGAR Operation "D" 00093 skip, ///< skipped region from the reference (the reference contains bases that have no corresponding base in the query sequence). Associated with CIGAR Operation "N" 00094 softClip, ///< Soft clip on the read (clipped sequence present in the query sequence, but not in reference). Associated with CIGAR Operation "S" 00095 hardClip, ///< Hard clip on the read (clipped sequence not present in the query sequence or reference). Associated with CIGAR Operation "H" 00096 pad ///< Padding (not in reference or query). Associated with CIGAR Operation "P" 00097 };
static bool Cigar::foundInQuery | ( | const CigarOperator & | op | ) | [inline, static] |
Return true if the specified operation is found in the query sequence, false if not.
Definition at line 254 of file Cigar.h.
References foundInQuery().
00255 { 00256 return(foundInQuery(op.operation)); 00257 }
static bool Cigar::foundInQuery | ( | char | op | ) | [inline, static] |
Return true if the specified operation is found in the query sequence, false if not.
static bool Cigar::foundInQuery | ( | Operation | op | ) | [inline, static] |
Return true if the specified operation is found in the query sequence, false if not.
Definition at line 219 of file Cigar.h.
References insert, match, mismatch, and softClip.
Referenced by foundInQuery(), SamRecord::shiftIndelsLeft(), SamFilter::softClip(), CigarHelper::softClipBeginByRefPos(), and CigarHelper::softClipEndByRefPos().
static bool Cigar::foundInReference | ( | const CigarOperator & | op | ) | [inline, static] |
Return true if the specified operation is found in the reference sequence, false if not.
Definition at line 212 of file Cigar.h.
References foundInReference().
00213 { 00214 return(foundInReference(op.operation)); 00215 }
static bool Cigar::foundInReference | ( | char | op | ) | [inline, static] |
Return true if the specified operation is found in the reference sequence, false if not.
static bool Cigar::foundInReference | ( | Operation | op | ) | [inline, static] |
Return true if the specified operation is found in the reference sequence, false if not.
Definition at line 177 of file Cigar.h.
References del, match, mismatch, and skip.
Referenced by foundInReference(), CigarHelper::softClipBeginByRefPos(), and CigarHelper::softClipEndByRefPos().
char Cigar::getCigarCharOp | ( | int32_t | expandedCigarIndex | ) |
Return the character code of the cigar operator associated with the specified expanded CIGAR index.
'?' is returned for an out of range index.
Definition at line 295 of file Cigar.cpp.
Referenced by getCigarCharOpFromQueryIndex(), getCigarCharOpFromRefOffset(), and getCigarCharOpFromRefPos().
00296 { 00297 // Check if the expanded cigar has been set yet 00298 if ((queryToRef.size() == 0) || (refToQuery.size() == 0)) 00299 { 00300 // Set the expanded cigar. 00301 setQueryAndReferenceIndexes(); 00302 } 00303 00304 // Check to see if the index is in range. 00305 if((expandedCigarIndex < 0) || 00306 ((uint32_t)expandedCigarIndex >= myExpandedCigar.length())) 00307 { 00308 return('?'); 00309 } 00310 return(myExpandedCigar[expandedCigarIndex]); 00311 }
char Cigar::getCigarCharOpFromQueryIndex | ( | int32_t | queryIndex | ) |
Return the character code of the cigar operator associated with the specified queryIndex.
'?' is returned for an out of range index.
Definition at line 314 of file Cigar.cpp.
References getCigarCharOp(), and getExpandedCigarIndexFromQueryIndex().
00315 { 00316 return(getCigarCharOp(getExpandedCigarIndexFromQueryIndex(queryIndex))); 00317 }
char Cigar::getCigarCharOpFromRefOffset | ( | int32_t | refOffset | ) |
Return the character code of the cigar operator associated with the specified reference offset.
'?' is returned for an out of range offset.
Definition at line 320 of file Cigar.cpp.
References getCigarCharOp(), and getExpandedCigarIndexFromRefOffset().
00321 { 00322 return(getCigarCharOp(getExpandedCigarIndexFromRefOffset(refOffset))); 00323 }
char Cigar::getCigarCharOpFromRefPos | ( | int32_t | refPosition, | |
int32_t | queryStartPos | |||
) |
Return the character code of the cigar operator associated with the specified reference position.
'?' is returned for an out of range reference position.
Definition at line 326 of file Cigar.cpp.
References getCigarCharOp(), and getExpandedCigarIndexFromRefPos().
00327 { 00328 return(getCigarCharOp(getExpandedCigarIndexFromRefPos(refPosition, queryStartPos))); 00329 }
void Cigar::getCigarString | ( | std::string & | cigarString | ) | const |
Set the passed in std::string to the string reprentation of the Cigar operations in this object.
Definition at line 36 of file Cigar.cpp.
00037 { 00038 using namespace STLUtilities; 00039 00040 std::vector<CigarOperator>::const_iterator i; 00041 00042 cigarString.clear(); // clear result string 00043 00044 // Progressively append the character representations of the operations to 00045 // the cigar string. 00046 for (i = cigarOperations.begin(); i != cigarOperations.end(); i++) 00047 { 00048 cigarString << (*i).count << (*i).getChar(); 00049 } 00050 }
void Cigar::getCigarString | ( | String & | cigarString | ) | const |
Set the passed in String to the string reprentation of the Cigar operations in this object.
Definition at line 52 of file Cigar.cpp.
Referenced by Dump(), and SamRecord::setCigar().
00053 { 00054 std::string cigar; 00055 00056 getCigarString(cigar); 00057 00058 cigarString = cigar.c_str(); 00059 00060 return; 00061 }
int32_t Cigar::getExpandedCigarIndexFromQueryIndex | ( | int32_t | queryIndex | ) |
Returns the index into the expanded cigar for the cigar associated with the specified queryIndex.
INDEX_NA returned if the index is out of range.
Definition at line 258 of file Cigar.cpp.
References INDEX_NA.
Referenced by getCigarCharOpFromQueryIndex().
00259 { 00260 // If the vectors aren't set, set them. 00261 if ((queryToRef.size() == 0) || (refToQuery.size() == 0)) 00262 { 00263 setQueryAndReferenceIndexes(); 00264 } 00265 if ((queryIndex < 0) || ((uint32_t)queryIndex >= queryToCigar.size())) 00266 { 00267 return(INDEX_NA); 00268 } 00269 return(queryToCigar[queryIndex]); 00270 }
int32_t Cigar::getExpandedCigarIndexFromRefOffset | ( | int32_t | refOffset | ) |
Returns the index into the expanded cigar for the cigar associated with the specified reference offset.
INDEX_NA returned if the offset is out of range.
Definition at line 273 of file Cigar.cpp.
References INDEX_NA.
Referenced by getCigarCharOpFromRefOffset(), and getExpandedCigarIndexFromRefPos().
00274 { 00275 // If the vectors aren't set, set them. 00276 if ((queryToRef.size() == 0) || (refToQuery.size() == 0)) 00277 { 00278 setQueryAndReferenceIndexes(); 00279 } 00280 if ((refOffset < 0) || ((uint32_t)refOffset >= refToCigar.size())) 00281 { 00282 return(INDEX_NA); 00283 } 00284 return(refToCigar[refOffset]); 00285 }
int32_t Cigar::getExpandedCigarIndexFromRefPos | ( | int32_t | refPosition, | |
int32_t | queryStartPos | |||
) |
Returns the index into the expanded cigar for the cigar associated with the specified reference position and queryStartPos.
INDEX_NA returned if the position is out of range.
Definition at line 288 of file Cigar.cpp.
References getExpandedCigarIndexFromRefOffset().
Referenced by getCigarCharOpFromRefPos().
00290 { 00291 return(getExpandedCigarIndexFromRefOffset(refPosition - queryStartPos)); 00292 }
void Cigar::getExpandedString | ( | std::string & | s | ) | const |
Sets the specified string to a valid CIGAR string of characters that represent the cigar with no digits (a CIGAR of "3M" would return "MMM").
The returned string is actually also a valid CIGAR string. In theory this makes it easier to parse some reads.
Definition at line 63 of file Cigar.cpp.
00064 { 00065 s = ""; 00066 00067 std::vector<CigarOperator>::const_iterator i; 00068 00069 // Progressively append the character representations of the operations to 00070 // the string passed in 00071 00072 for (i = cigarOperations.begin(); i != cigarOperations.end(); i++) 00073 { 00074 for (uint32_t j = 0; j<(*i).count; j++) s += (*i).getChar(); 00075 } 00076 return; 00077 }
int Cigar::getExpectedQueryBaseCount | ( | ) | const |
Return the length of the read that corresponds to the current CIGAR string.
For validation, we should expect that a sequence read in a SAM file will be the same length as the value returned by this method.
Example: 3M2D3M describes a read with three bases matching the reference, then skips 2 bases, then has three more bases that match the reference (match/mismatch). In this case, the read length is expected to be 6.
Example: 3M2I3M describes a read with 3 match/mismatch bases, two extra bases, and then 3 more match/mistmatch bases. The total in this example is 8 bases.
Definition at line 95 of file Cigar.cpp.
References insert, match, mismatch, and softClip.
Referenced by SamValidator::isValidCigar(), and SamFilter::softClip().
00096 { 00097 int matchCount = 0; 00098 std::vector<CigarOperator>::const_iterator i; 00099 for (i = cigarOperations.begin(); i != cigarOperations.end(); i++) 00100 { 00101 switch (i->operation) 00102 { 00103 case match: 00104 case mismatch: 00105 case softClip: 00106 case insert: 00107 matchCount += i->count; 00108 break; 00109 default: 00110 // we only care about operations that are in the query sequence. 00111 break; 00112 } 00113 } 00114 return matchCount; 00115 }
int Cigar::getExpectedReferenceBaseCount | ( | ) | const |
Return the number of bases in the reference that this CIGAR "spans".
When doing range checking, we occassionally need to know how many total bases the CIGAR string represents as compared to the reference.
Examples: 3M2D3M describes a read that overlays 8 bases in the reference. 3M2I3M describes a read with 3 bases that match the reference, two additional bases that aren't in the reference, and 3 more bases that match the reference, so it spans 6 bases in the reference.
Definition at line 120 of file Cigar.cpp.
References del, match, mismatch, and skip.
Referenced by SamTags::createMDTag().
00121 { 00122 int matchCount = 0; 00123 std::vector<CigarOperator>::const_iterator i; 00124 for (i = cigarOperations.begin(); i != cigarOperations.end(); i++) 00125 { 00126 switch (i->operation) 00127 { 00128 case match: 00129 case mismatch: 00130 case del: 00131 case skip: 00132 matchCount += i->count; 00133 break; 00134 default: 00135 // we only care about operations that are in the reference sequence. 00136 break; 00137 } 00138 } 00139 return matchCount; 00140 }
uint32_t Cigar::getNumOverlaps | ( | int32_t | start, | |
int32_t | end, | |||
int32_t | queryStartPos | |||
) |
Return the number of bases that overlap the reference and the read associated with this cigar that falls within the specified region.
start | : inclusive 0-based start position (reference position) of the region to check for overlaps in (-1 indicates to start at the beginning of the reference.) | |
end | : exclusive 0-based end position (reference position) of the region to check for overlaps in (-1 indicates to go to the end of the reference.) | |
queryStartPos | : 0-based leftmost mapping position of the first matcihng base in the query. |
Definition at line 334 of file Cigar.cpp.
References getRefOffset().
Referenced by SamRecord::getNumOverlaps().
00336 { 00337 // Get the overlap info. 00338 if ((queryToRef.size() == 0) || (refToQuery.size() == 0)) 00339 { 00340 setQueryAndReferenceIndexes(); 00341 } 00342 00343 // Get the start and end offsets. 00344 int32_t startRefOffset = 0; 00345 // If the specified start is more than the queryStartPos, set 00346 // the startRefOffset to the appropriate non-zero value. 00347 // (if start is <= queryStartPos, than startRefOffset is 0 - it should 00348 // not be set to a negative value.) 00349 if (start > queryStartPos) 00350 { 00351 startRefOffset = start - queryStartPos; 00352 } 00353 00354 int32_t endRefOffset = end - queryStartPos; 00355 if (end == -1) 00356 { 00357 // -1 means that the region goes to the end of the refrerence. 00358 // So set endRefOffset to the max refOffset + 1 which is the 00359 // size of the refToQuery vector. 00360 endRefOffset = refToQuery.size(); 00361 } 00362 00363 00364 // if endRefOffset is less than 0, then this read does not fall within 00365 // the specified region, so return 0. 00366 if (endRefOffset < 0) 00367 { 00368 return(0); 00369 } 00370 00371 // Get the overlaps for these offsets. 00372 // Loop through the read counting positions that match the reference 00373 // within this region. 00374 int32_t refOffset = 0; 00375 int32_t numOverlaps = 0; 00376 for (unsigned int queryIndex = 0; queryIndex < queryToRef.size(); 00377 queryIndex++) 00378 { 00379 refOffset = getRefOffset(queryIndex); 00380 if (refOffset > endRefOffset) 00381 { 00382 // Past the end of the specified region, so stop checking 00383 // for overlaps since there will be no more. 00384 break; 00385 } 00386 else if ((refOffset >= startRefOffset) && (refOffset < endRefOffset)) 00387 { 00388 // within the region, increment the counter. 00389 ++numOverlaps; 00390 } 00391 } 00392 00393 return(numOverlaps); 00394 }
int32_t Cigar::getQueryIndex | ( | int32_t | refPosition, | |
int32_t | queryStartPos | |||
) |
Return the query index or INDEX_NA associated with the specified reference offset when the query starts at the specified reference position.
Definition at line 240 of file Cigar.cpp.
References INDEX_NA.
00241 { 00242 // If the vectors aren't set, set them. 00243 if ((queryToRef.size() == 0) || (refToQuery.size() == 0)) 00244 { 00245 setQueryAndReferenceIndexes(); 00246 } 00247 00248 int32_t refOffset = refPosition - queryStartPos; 00249 if ((refOffset < 0) || ((uint32_t)refOffset >= refToQuery.size())) 00250 { 00251 return(INDEX_NA); 00252 } 00253 00254 return(refToQuery[refOffset]); 00255 }
int32_t Cigar::getQueryIndex | ( | int32_t | refOffset | ) |
Return the query index associated with the specified reference offset or INDEX_NA based on this cigar.
Definition at line 202 of file Cigar.cpp.
References INDEX_NA.
Referenced by PileupElementBaseQual::addEntry(), SamRecordHelper::checkSequence(), and SamTags::createMDTag().
00203 { 00204 // If the vectors aren't set, set them. 00205 if ((queryToRef.size() == 0) || (refToQuery.size() == 0)) 00206 { 00207 setQueryAndReferenceIndexes(); 00208 } 00209 if ((refOffset < 0) || ((uint32_t)refOffset >= refToQuery.size())) 00210 { 00211 return(INDEX_NA); 00212 } 00213 return(refToQuery[refOffset]); 00214 }
int32_t Cigar::getRefOffset | ( | int32_t | queryIndex | ) |
Return the reference offset associated with the specified query index or INDEX_NA based on this cigar.
Definition at line 187 of file Cigar.cpp.
References INDEX_NA.
Referenced by SamQuerySeqWithRefIter::getNextMatchMismatch(), getNumOverlaps(), SamQuerySeqWithRef::seqWithEquals(), and SamQuerySeqWithRef::seqWithoutEquals().
00188 { 00189 // If the vectors aren't set, set them. 00190 if ((queryToRef.size() == 0) || (refToQuery.size() == 0)) 00191 { 00192 setQueryAndReferenceIndexes(); 00193 } 00194 if ((queryIndex < 0) || ((uint32_t)queryIndex >= queryToRef.size())) 00195 { 00196 return(INDEX_NA); 00197 } 00198 return(queryToRef[queryIndex]); 00199 }
int32_t Cigar::getRefPosition | ( | int32_t | queryIndex, | |
int32_t | queryStartPos | |||
) |
Return the reference position associated with the specified query index or INDEX_NA based on this cigar and the specified queryStartPos which is the leftmost mapping position of the first matching base in the query.
Definition at line 217 of file Cigar.cpp.
References INDEX_NA.
Referenced by SamFilter::softClip().
00218 { 00219 // If the vectors aren't set, set them. 00220 if ((queryToRef.size() == 0) || (refToQuery.size() == 0)) 00221 { 00222 setQueryAndReferenceIndexes(); 00223 } 00224 if ((queryIndex < 0) || ((uint32_t)queryIndex >= queryToRef.size())) 00225 { 00226 return(INDEX_NA); 00227 } 00228 00229 if (queryToRef[queryIndex] != INDEX_NA) 00230 { 00231 return(queryToRef[queryIndex] + queryStartPos); 00232 } 00233 return(INDEX_NA); 00234 }
bool Cigar::hasIndel | ( | ) |
Return whether or not the cigar has indels (insertions or delections).
Definition at line 398 of file Cigar.cpp.
00399 { 00400 for(unsigned int i = 0; i < cigarOperations.size(); i++) 00401 { 00402 if((cigarOperations[i].operation == insert) || 00403 (cigarOperations[i].operation == del)) 00404 { 00405 // Found an indel, so return true. 00406 return(true); 00407 } 00408 } 00409 // Went through all the operations, and found no indel, so return false. 00410 return(false); 00411 }
static bool Cigar::isClip | ( | const CigarOperator & | op | ) | [inline, static] |
static bool Cigar::isClip | ( | char | op | ) | [inline, static] |
static bool Cigar::isClip | ( | Operation | op | ) | [inline, static] |
Return true if the specified operation is a clipping operation, false if not.
Definition at line 261 of file Cigar.h.
References hardClip, and softClip.
Referenced by isClip(), SamFilter::softClip(), and CigarHelper::softClipEndByRefPos().
static bool Cigar::isMatchOrMismatch | ( | const CigarOperator & | op | ) | [inline, static] |
Return true if the specified operation is a match/mismatch operation, false if not.
Definition at line 313 of file Cigar.h.
References isMatchOrMismatch().
00314 { 00315 return(isMatchOrMismatch(op.operation)); 00316 }
static bool Cigar::isMatchOrMismatch | ( | Operation | op | ) | [inline, static] |
Return true if the specified operation is a match/mismatch operation, false if not.
Definition at line 298 of file Cigar.h.
References match, and mismatch.
Referenced by isMatchOrMismatch(), and SamRecord::shiftIndelsLeft().
bool Cigar::operator== | ( | Cigar & | rhs | ) | const |
const int32_t Cigar::INDEX_NA = -1 [static] |
Value associated with an index that is not applicable/does not exist, used for converting between query and reference indexes/offsets when an associated index/offset does not exist.
Definition at line 492 of file Cigar.h.
Referenced by PileupElementBaseQual::addEntry(), SamRecordHelper::checkSequence(), SamTags::createMDTag(), getExpandedCigarIndexFromQueryIndex(), getExpandedCigarIndexFromRefOffset(), SamQuerySeqWithRefIter::getNextMatchMismatch(), getQueryIndex(), getRefOffset(), getRefPosition(), SamQuerySeqWithRef::seqWithEquals(), and SamQuerySeqWithRef::seqWithoutEquals().